Microbiological Assay of Folic Acid Content in Some Selected Bangladeshi Food Stuffs
نویسندگان
چکیده
منابع مشابه
Microbiological Assay of Folic Acid Activity in Human Serum.
A method is described for the microbiological assay of folic acid activity in serum with Lactobacillus casei as test organism and a modified medium in which the organism gives a greater growth response than in media previously detailed. The results of experiments carried out to validate the use of this medium are shown. In 94 control subjects levels of folic acid activity in the serum ranged fr...
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Folate-depleted, cryoprotected preparations of Lactobacillus casei are stable for at least eight months at -18 degrees C, capable of reproducible growth and suitable as a ready source of inoculum for measurement of folates in physiological fluids. Cryoprotected microorganisms can be a commercially available laboratory reagent, thus simplifying and further standardizing the microbiological assay...
متن کاملThe use of conjugase preparations in the microbiological assay of folic acid.
Taka-diastase (1) and a preparation of hog kidney enzyme (2) have been used routinely to liberate folic acid from its conjugates in the microbiological determination of the vitamin (3-6). However, Olson et al. (7) reported recently that taka-diastase and certain proteolytic enzymes are of doubtful value in releasing the vitamin from plant tissues. Hog kidney conjugase also does not completely l...
متن کاملUse of glycerol-cryoprotected Lactobacillus casei for microbiological assay of folic acid.
A simple procedure for preparing glycerol-cryoprotected Lactobacillus casei cultures has been developed. L. casei grown in medium supplemented with low concentrations of folic acid (0.3 micrograms/L) is diluted with an equal volume of glycerol (800 mL/L) and stored at -20 degrees C. Growth response of the glycerol-cryoprotected L. casei to low concentrations of folic acid exceeded that of cult...
متن کاملFolic Acid Absorption Studies a Comparison of Two Microbiological Assay Techniques.
In the investigation of malabsorption syndromes and folic acid deficiency, the folic acid absorption test may be used to determine the degree of absorption of folic acid from the intestine and also to measure the rate of clearance from the serum . The test is based on loading the patient with subcutaneous folic acid followed by an oral dose of this vitamin. Samples of blood are withdrawn at sui...
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ژورنال
عنوان ژورنال: International Journal of Biology
سال: 2015
ISSN: 1916-968X,1916-9671
DOI: 10.5539/ijb.v7n2p35